| 1. | Development and application of oligonucleotide microarray for detecting rifampin - resistant mycobacterium tuberculosis strains 应用微孔噬菌体扩增法检测结核分枝杆菌对利福平的敏感性 |
| 2. | Reliable detection of rifampin - resistance of mycobacterium tuberculosis strains by using a specialized oligonucleotide microarray 检测耐利福平结核分枝杆菌寡核苷酸芯片的研制及应用 |
| 3. | Oligonucleotide microarray technology is a powerful data - mining platform and has been widely applied in biosciences 摘要寡核苷酸芯片技术是一种高通量发掘和采集生物信息的强大技术平合,目前已广泛应用于生物科学领域。 |
| 4. | On the basis of the first step of amplifying pcr , it has been hybridized with oligonucleotide microarray in order to analyze whether the amplified pcr product possess antimicrobial agent resistance or not 摘要在第一步耐药性靶基因得到扩增的基础上,通过与芯片进行杂交来分析所扩增的pcr产物是否是已经发生了突变而能导致抗药性的基因。 |
| 5. | To improve the performance of assays on the oligonucleotide microarray , the factors that influence the hybridization effects such as surface chemistry , probe size , spacer length , hybridization conditions etc were intensely studied and optimized 为改善寡核苷酸芯片的分析性能,对影响芯片杂交结果的因素,如片基表面的化学处理、探针的长度、间隔臂的长度、杂交条件等,进行了深入的研究和优化。 |
| 6. | 5 . prepare the oligonucleotide microarray add 16 prepared probes into 384 - pole plate , meantime , add p2 , low homologic probes and probe buffer in the same concentration as positive control , blank control and negative control respectively . spot microarray as designed by the spotting machine 5 .寡核昔酸微阵列的制备以同等浓度的反义链引物咫作为阳性对照,以探针缓冲液作为空白对照,以无关探针作为阴性对照,与制备好的16条寡核昔酸探针,同时加人384孔板。 |